International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE
Karen L. Laurie, Othmar G. Engelhardt, John Wood, Alan Heath, Jacqueline M. Katz, Malik Peiris, Katja Hoschler, Olav Hungnes, Wenqing Zhang, Maria D. Van Kerkhove, and the CONSISE Laboratory Working Group participants
Clinical and Vaccine Immunology 2015 Jun 24. pii: CVI.00278-15. [Epub ahead of print]
This manuscript describes the CONSISE Laboratory Working Group's laboratory comparison of the 2-day ELISA-based and 3-day haemagglutination(HA)-based MN Assays. We established CONSISE Consensus protocols for the assays detecting antibodies to A(H1N1)pdm09 virus, identifying the required and recommended parameters, which are specified in the manuscript. CONSISE laboratories performed both 2-day and 3-day assays on panels of sera and the sample titres were compared within each laboratory. Antibodies to A(H1N1)pdm09, A(H3N2) and A(H5N1) viruses were detected. The results were comparable for both assays, indicating that either the 2-day ELISA or the 3-day HA MN assays may be used to detect antibodies to these influenza A subtypes. The participating laboratories have now aligned their laboratory methodologies to the CONSISE consensus protocols.
The manuscript is open access. The PubMed link is: http://www.ncbi.nlm.nih.gov/pubmed/26108286